Immunoblotting was performed with the following primary antibodies: anti-GPX4 (Abcam, catalog ab125066, 1:3000), anti-ACSL4 (Santa Cruz, catalog sc-365230, 1:1000), anti-LPCAT3 (Abcam, catalog ab232958, 1:500), anti-fibronectin (Abcam, catalog ab45688, 1:5000), anti-collagen-I (Proteintech, catalog 14695-1-AP, 1:1000), and anti--SMA (Abcam, catalog ab124964, 1:2000)
doi: 10.1038/srep37744 (2016)
Presented for research literature review only Wessells H et al
Rather than reflecting a single hormonal deficiency, these conditions involve widespread disruptions in how insulin signals are received and processed across multiple tissues
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They occur when bacteria grow in the bladder or urethra